G005951
HPLC Analysis of Fusion Protein on Zenix®-C SEC-300 vs. Zenix® SEC-300
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777033)
column: Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777092)
mobile phase: 150 mM sodium phosphate buffer, pH 7.0 + 200 mM NaCl
flow rate: 0.5 mL/min
column temp.: 25 °C
detector: UV, 214 nm
injection: 10 μL
sample: 1 mg/mL fusion protein, MW 170 kD, pl 6.8-7.0
suitability
application for HPLC
application(s)
life science and biopharma
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