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G005921

GC Analysis of PCBs in Whole Cow Milk (Spiked) on SLB®-5ms (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis

suitable for GC, application for SPE

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technique(s)

gas chromatography (GC): suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: 10 g whole cow′s milk
extraction tube: Supel™ QuE Non-buffered tube (4 g magnesium sulfate, 1 g sodium chloride) (55294-U)
clean-up tube: Supel™ QuE Z-Sep 150 mg, magnesium sulfate (150 mg, part no. 63135) (55299-U)
column: SLB-5ms, 20 m x 0.18 mm I.D., 0.18 μm (28564-U)
oven: 75 °C (1 min), 12 °C/min to 340 °C (10 min)
inj. temp.: 250 °C
detector: μ-ECD, 340 °C
carrier gas: hydrogen, 1.2 mL/min, constant
injection: 1 μL splitless (0.75 min)
liner: 4 mm I.D. FocusLiner™ with taper

suitability

application for SPE

application(s)

food and beverages

General description

Reference: Stenerson, K.; Ye, M.; Halpenny, M., New Analytical Tools for the Determination of Persistent Organic Pollutants (POPs) in Fatty Food and Beverage Matrices Using QuEChERS Extraction/Cleanup and Gas Chromatography (GC) Analysis. Pittcon 2013. Sigma-Aldrich/Supelco publication T413034

Analysis Note

This application demonstrates the use of dispersive SPE or "QuEChERS" for the extraction and cleanup of whole cow′s milk for GC-ECD analysis of polychlorinated biphenyl (PCBs). Z-Sep sorbent was used in the cleanup step and found to reduce background and yield recoveries >70% for all PCB congeners evaluated except no. 209, which was recovered at 68%.
Extraction solvent: acetone:hexane (1:1), 20 mL
Clean-up volume: 1 mL

Legal Information

FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC

Certificates of Analysis (COA)

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