G005904
HPLC Analysis of Yohimbe Alkaloids on Ascentis® Express F5, HILIC Mode
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express F5, 3 cm x 3.0 mm I.D., 2.7 μm particles (53574-U)
mobile phase: 2 mM ammonium acetate in 10:90 water:acetonitrile, pH to 6.0 with acetic acid
flow rate: 0.5 mL/min
pressure: 1100 psi (76 bar)
column temp.: 35 °C
detector: UV, 275 nm
injection: 2 μL
sample: 100 ug/mL each in methanol
suitability
application for HPLC
application(s)
vitamins, nutraceuticals, and natural products
Legal Information
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analytical column
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