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G005879

LC/MS/MS Analysis of Epinephrine, Metanephrine and Metabolites on Ascentis® Express OH5

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53757-U)
mobile phase: [A] 5 mM ammonium formate; [B] 5 mM ammonium  formate in acetonitrile; (15:85, A:B)
flow rate: 0.3 mL/min
pressure: 1189 psi (82 bar)
column temp.: 35 °C
detector: MS ESI(+), 100-1000 m/z
injection: 1 μL
sample: 300 ng/mL each in acetonitrile

suitability

application for HPLC

application(s)

clinical

Analysis Note

HILIC mode on Ascentis Express OH5 provided rapid separation of neurotransmitters in highly MS-compatible mobile phases. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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