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G005802

HPLC Analysis of Polar Compounds on Ascentis® Express OH5 (HILIC Mode)

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express OH5, 10 cm x 3 mm I.D., 2.7 μm particles (53769-U)
mobile phase: 5 mM ammonium formate (95:5 acetonitrile:water) pH 6.8
flow rate: 0.6 mL/min
pressure: 965 psi (66.5 bar)
column temp.: 30 °C
detector: UV, 254 nm
injection: 1 μL
sample: 100 μg/mL in 25:75, water:methanol

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Analysis Note

The impact of partitioning and ion exchange in HILIC separations is demonstrated using a mixture of acidic, neutral, and basic polar compounds in this Figure. All three analytes have similar log D values, both acid and base are ionized at mobile phase pH.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

analytical column

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