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G005791

LC/MS Analysis of L-Methionine Sulfoximine and Sulfone on Ascentis® Express OH5

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express OH5, 10 cm x 3.0 mm I.D., 2.7 μm particles (53769-U)
mobile phase: [A] acetonitrile; [B] water with 0.1% formic acid, pH 3.5 with ammonium formate; (75:25, A:B)
flow rate: 0.4 mL/min
pressure: 1350 ppi (93 bar)
column temp.: 25 °C
detector: MS, ESI (+), TIC, m/z 150-300
injection: 5 μL

suitability

application for HPLC

application(s)

chemicals and industrial polymers

Analysis Note

L-methionine sulfoximine and L-buthionine sulfoximine are used in are used to prevent additional enzyme activity in Chinese hampster ovary (CHO) cell lines with supplemental Glutamine Synthase (GS). It is of interest to be able to monitor both purity of such molecules in order to control addition to cell media as well as for the assay of parent molecules during use. The analytes are highly polar and thus should be amenable to HILIC-LC-MS analysis. This first installment investigates the ability of Ascentis Express HILIC and Ascentis Express OH5 stationary phases for the separation of the methionine analog and related compounds as well as methionine and buthionine separations.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    L-Methionine sulfone
    L-Methionine sulfoximine, suitable for cell culture

analytical column

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standard

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