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G005753

HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection)

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)
mobile phase: [A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)
flow rate: 0.6 mL/min
pressure: 812 psi (56 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 2 μL
sample: Dilute 1:9 in acetonitrile

suitability

application for HPLC

application(s)

food and beverages

Analysis Note

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UVand ELSD detectors in series, this chromatogram being from the UV.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Caffeine
    Benzoic acid
    Sorbic acid

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