G005716
HPLC Analysis of Acetaminophen, Caffeine, Chlorpheniramine, Ibuprofen, Naproxen, Phenylephrine, and Pseudoephedrine on Ascentis® Express C18
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
mobile phase: [A] 5 mM ammonium phosphate monobasic, pH 2.0 with phosphoric acid: acetonitrile (98:2); [B] acetonitrile;
gradient: 0 to 68% B in 3 min; held at 68% B for 2 min
flow rate: 0.4 mL/min
column temp.: 35 °C
detector: UV, 210 nm
injection: 1 μL
sample: 100 μg/mL in 95:5, water: methanol
suitability
application for HPLC
application(s)
pharmaceutical (small molecule)
Analysis Note
Legal Information
Analyte
analytical column
standard
Certificates of Analysis (COA)
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