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Key Documents

G005711

LC/MS Analysis of Omeprazole and Metabolites in Plasma on Ascentis® Express RP Amide after Sample Prep using HybridSPE®-Phospholipid

application for HPLC, application for SPE

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1 KIT
CN¥5,713.28

CN¥5,713.28

Available to ship onApril 27, 2025Details

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1 KIT
CN¥5,713.28

About This Item

CN¥5,713.28

Available to ship onApril 27, 2025Details

technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: rat plasma spiked with omeprazole, phenacetin and metabolites at 2 μg/mL
SPE well plate: HybridSPE-Phospholipid, 96-well plate, 15 mg/0.8 mL (575656-U)
sample addition: 100 μL plasma to each well, followed by 300 mL of 1% formic acid in acetonitrile
elution: apply vacuum
eluate post-treatment: eluate was collected, evaporated, and reconstituted in mobile phase.
column: Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.7 μm particles (53911-U)
mobile phase: [A] water, 10 mM ammonium formate, pH 3.4; [B] methanol, 10 mM ammonium formate, pH 3.4; (65:35, A:B)
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+), m/z = 50-400
injection: 1 μL

suitability

application for HPLC
application for SPE

application(s)

clinical

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Gene Information

mouse ... Il10(16153)

Gene Information

rat ... Il10(25325)

Gene Information

mouse ... Il11(16156)

Gene Information

mouse ... Il15(16168)

species reactivity

mouse

species reactivity

rat

species reactivity

mouse

species reactivity

mouse

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 45 pg/mL
standard curve range: 78.13-5000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1000 pg/mL
standard curve range: 1.85-450 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 500 pg/mL
standard curve range: 0.33-80 ng/mL

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

Application

This application demonstrates the suitability of HybridSPE and the Ascentis Express RP Amide for the analysis of omeprazole metabolites.

Analysis Note

Sample was vorlexed and centrifuged for 2 minutes at 15000 rpm. Supemate was then passed through HybridSPE 96 well plate. Elutent was collected, evaporated and reconstituted in mobile phase.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    5-Hydroxyomeprazole
    Phenacetin
    Omeprazole
    Omeprazole sulfone

SPE tube or plate

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Description
Pricing

analytical column

Product No.
Description
Pricing

standard

Product No.
Description
Pricing

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