G005711
LC/MS Analysis of Omeprazole and Metabolites in Plasma on Ascentis® Express RP Amide after Sample Prep using HybridSPE®-Phospholipid
application for HPLC, application for SPE
About This Item
technique(s)
HPLC: suitable
solid phase extraction (SPE): suitable
test parameters
sample preparation: SPE (Solid Phase Extraction)
sample/matrix: rat plasma spiked with omeprazole, phenacetin and metabolites at 2 μg/mL
SPE well plate: HybridSPE-Phospholipid, 96-well plate, 15 mg/0.8 mL (575656-U)
sample addition: 100 μL plasma to each well, followed by 300 mL of 1% formic acid in acetonitrile
elution: apply vacuum
eluate post-treatment: eluate was collected, evaporated, and reconstituted in mobile phase.
column: Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.7 μm particles (53911-U)
mobile phase: [A] water, 10 mM ammonium formate, pH 3.4; [B] methanol, 10 mM ammonium formate, pH 3.4; (65:35, A:B)
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+), m/z = 50-400
injection: 1 μL
suitability
application for HPLC
application for SPE
application(s)
clinical
Application
Analysis Note
Legal Information
Analyte
SPE tube or plate
analytical column
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Sorry, we don't have COAs for this product available online at this time.
If you need assistance, please contact Customer Support.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service