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G005696

LC/MS Analysis of Erythromycin Analogs on Ascentis® Express HILIC

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase: [A] 100 mM ammonium formate, pH 3.0 (titrated with formic acid); [B] acetonitrile; (10:90, A:B)
flow rate: 1.0 mL/min
pressure: 1247 psi (86 bar)
column temp.: 35 °C
detector: ESI(+); SIR
injection: 5 μL

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Analysis Note

This application shows the suitability of Ascentis Express Silica columns for the analysis of erythromycin analogs in aqueous normal phase/HILIC mode of chromatography. Other relatively hydrophobic aminoglycosides have also been shown to separate under these conditions.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Erythromycin ethyl succinate
    Erythromycin estolate
    Erythromycin

analytical column

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mobile phase component

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standard

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