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G005678

HPLC Analysis of Beverage Additives in Diet Cola on Ascentis® Express RP-Amide

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 10 cm x 3.0 mm I.D., 2.7 μm particles (53918-U)
mobile phase: [A] 20 mM ammonium acetate, pH 4.7 with acetic acid; [B] acetonitrile; (92:8, A:B)
flow rate: 0.6 mL/min
column temp.: 40 °C
detector: UV, 214 nm
injection: 1 μL
sample: 500 - 1000 μg/mL in 95:5, buffer: acetonitrile

suitability

application for HPLC

application(s)

food and beverages

Analysis Note

This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of common drink additives used in diet cola.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Caffeine
    Benzoic acid
    Asp-Phe methyl ester

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