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G005673

HPLC Analysis of Benzodiazepines on Ascentis® Express C18, Acetonitrile Mobile Phase

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] water; [B] acetonitrile; (66:34, A:B)
flow rate: 0.6 mL/min
column temp.: 35 °C
detector: UV, 250 nm
injection: 2 μL
sample: 0.1 g/L each in 20% methanol

suitability

application for HPLC

application(s)

clinical
pharmaceutical (small molecule)

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description

    Oxazepam
    Clonazepam
    Alprazolam
    Desmethyldiazepam
    Diazepam

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