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Key Documents

G005665

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18

application for HPLC

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12 UNITS
CN¥1,072.68

CN¥1,072.68


Estimated to ship onJune 03, 2025Details



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12 UNITS
CN¥1,072.68

About This Item


CN¥1,072.68


Estimated to ship onJune 03, 2025Details


technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: [A] 10 mM ammonium formate, pH 3.0; [B] acetonitrile; (90:10, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 280 nm
injection: 5 μL

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

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This Item
13191013192095299
assay

34.5-36.5%

assay

-

assay

-

assay

≥35% (RT)

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

100

solubility

diethyl ether: soluble, water: soluble, petroleum ether: insoluble

solubility

-

solubility

-

solubility

-

reaction suitability

reagent type: oxidant

reaction suitability

reagent type: oxidant

reaction suitability

reagent type: oxidant

reaction suitability

reagent type: oxidant

density

1.130 g/cm3

density

1.13 g/cm3

density

1.13 g/cm3

density

1.13 g/mL at 20 °C

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

2-8°C

Analysis Note

This application demonstrates the suitability of the Ascentis C18 for the analysis of aminopterin, folic acid and methotrexate. Structures along with the optimized chromatogram obtained on the Ascentis Express C18 are presented

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description

    Aminopterin
    Folic acid
    Methotrexate

standard

Product No.
Description
Pricing

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