HPLC Analysis of Nucleosides on Ascentis^® Express OH5 (HILIC Mode)

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53757-U)
mobile phase: [A] 5 mM ammonium acetate, pH 5.0 with acetic acid in 95:5, acetonitrile:water; [B] 5 mM ammonium acetate, pH 5.0 with acetic acid in 80:20, acetonitrile:water
gradient: 0% B held for 1 min; to 100% B in 10 min; held at 100% B for 1 min
flow rate: 0.3 mL/min
column temp.: 25 °C
detector: UV, 250 nm
injection: 2 μL
sample: 10 - 100 μg/mL in 95:5, acetonitrile:water (pH of buffer stock (in water) was adjusted before further dilution with water and/or acentonitrile)

suitability

application for HPLC

application(s)

life science and biopharma