LC/MS/MS Analysis of Carbendazim in Orange Juice (Spiked) on Ascentis^® Express C18 after Supel^™ QuE PSA/C18 QuEChERS Cleanup

technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: Dispersive (QuEChERS)
sample/matrix: orange juice spiked with carbendazim at 1 ppb
extraction tube: Supel^™ QuE Citrate (EN) Tube (55227-U)
extraction process: add 10 mL of orange juice with pulp to a 50 mL empty extraction tube (55248-U); add carbendazim at 1 ppb; add 10 mL acetonitrile; (shake for 1 minute; add contents of citrate extraction tube (55227-U); shake for 1 min; centrifuge at 3200 rpm for 5 minutes)
clean-up tube: Supel-Q^™ PSA/C18 (55288-U)
clean-up process: transfer 0.7 mL of the acetonitrile layer to the PSA/C18 cleanup tube (55288-U); shake 1 minute; (centrifuge for 3 minutes at 3400 rpm; remove 0.25 mL of the supernatant; mix with 0.25 mL water for LC-MS analysis)
column: Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
mobile phase: [A] 10 mM ammonium acetate in water; [B] 10 mM ammonium acetate in methanol
gradient: 0-1 min: 30% B; 1.5-3.5 min: 100% B; 3.5-7 min: 30% B
flow rate: 0-1 min: 0.3 mL/min; 1.5-7 min: 0.5 mL/min
column temp.: 30 °C
detector: MS, ESI(+), MRM, m/z 192/160, 192/132
injection: 5 μL

suitability

application for HPLC
application for SPE

application(s)

food and beverages