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G005596

HPLC Analysis of Polyphenols in Nero d′Avola Red Wine on Discovery® HS C18 (UV 354 nm)

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

sample/matrix: Nero d’Avola wine sample filtered prior injection through a 0.45 μm nylon membrane
column: Discovery HS C18, 15 cm x 2.1 mm I.D., 3 μm particles (569255-U)
mobile phase: [A] water, pH 3 with fomic acid; [B] Acetonitrile, pH 3 with fomic acid
flow rate: 0.2 mL/min
column temp.: 30 °C
detector: UV, 354 nm
injection: 5 μL

suitability

application for HPLC

application(s)

food and beverages

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description

    Caftaric acid, analytical standard
    Rutin hydrate, ≥94% (HPLC), powder
    Isoquercitrin, primary reference standard

analytical column

standard


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