G005530
HPLC Analysis of Bisphenol A in Water on Ascentis® Express C18 after SPE using Supelclean™ ENVI-18
application for HPLC
technique(s)
HPLC: suitable
test parameters
SPE tube/cartridge: Supelclean ENVI-18, 500 mg/6 mL glass SPE tube (54331-U)
condition: 1 mL 1% formic acid in acetonitrile, then 1 mL water
sample: 5 mL spiked water blank, concentration of 0.2 μg/mL
elution: 2 mL 1% formic acid in acetonitrile
eluate post-treatment: 1 mL aliquot of eluent, and evaporate/reconstitute to 0.5 mL in acetonitrile
column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] water; [B] acetonitrile; (60:40, A:B)
flow rate: 0.4 mL/min
pressure: 3270 psi (256 bar)
column temp.: 35 °C
detector: UV, 230 nm
injection: 1 μL
suitability
application for HPLC
application(s)
environmental
Analysis Note
Legal Information
Analyte
SPE tube or plate
analytical column
standard
Certificates of Analysis (COA)
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