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G005441

HPLC Analysis of Aflatoxins on Ascentis® Express C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] 5 mM ammonium formate, pH 2.5 (titrated with formic acid); [B] methanol
gradient: 5 to 100% B in 10 min
flow rate: 0.4 mL/min
pressure: 4714 psi (325 bar)
column temp.: 35 °C
detector: APCI (+)
injection: 5 μL
sample: 0.15 - 30 mg/L in 90:10, water:acetonitrile

suitability

application for HPLC

application(s)

food and beverages

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of various toxins.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Deoxynivalenol
    Aflatoxin B1
    Aflatoxin B2
    Aflatoxin G1
    Aflatoxin G2
    Aflatoxin M1
    Fumonisin B1 solution
    Ochratoxin A
    Fumonisin B2 solution
    Zearalenone
    See All (10)

analytical column

Product No.
Description
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