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G004734

HPLC Analysis of Methadone Enantiomers and Methadone Metabolite EDDP on Astec® CYCLOBOND I 2000 HP-RSP

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)
mobile phase: [A] 0.1% acetic acid; [B] acetonitrile; (80:20, A:B)
flow rate: 0.8 mL/min
detector: UV, 292 nm

suitability

application for HPLC

application(s)

forensics and toxicology

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CYCLOBOND is a trademark of Sigma-Aldrich Co. LLC

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