LC/MS Analysis of Clenbuterol In Plasma on Astec^® CHIROBIOTIC^® T with Phospholipid Removal using HybridSPE^® Phospholipid

technique(s)

LC/MS: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: rat plasma spiked with clenbuterol enantiomers at 10 ng/mL
SPE well plate: HybridSPE-Precipitation 96-well Plate, 50 mg/well (575656-U)
sample addition: 100 μL spiked rat plasma followed by 300 μL 1% formic acid in acetonitrile. Mix by vortexing the HybridSPE-PPT plate briefly.
elution: apply vacuum
column: CHIROBIOTIC T, 10 cm x 2.1 mm I.D., 5 μm particles (12018AST)
mobile phase: 10 mM ammonium formate in methanol
flow rate: 0.3 mL/min
column temp.: 30 °C
detector: ABI 3200 QT; ESI(+), MRM: 184/104 m/z (phospholipids); 277.2/203.1 m/z (clenbuterol)
injection: 10 μL

suitability

application for LC-MS
application for SPE

application(s)

clinical
forensics and toxicology
pharmaceutical (small molecule)