G003764
HPLC Analysis of Furosemide (Lasix) in Horse Serum on Discovery® C18 after SPE using Discovery® DSC-18
application for SPE, application for HPLC
technique(s)
HPLC: suitable
solid phase extraction (SPE): suitable
test parameters
sample preparation: SPE (Solid Phase Extraction)
sample/matrix: acidified horse serum spiked with indapamide at 10 mg/mL
SPE tube/cartridge: Discovery DSC-18, 50 mg/1 mL (52601-U)
condition: 1 mL methanol; 1 mL 10 mM potassium phosphate, pH 3
sample addition: 1 mL
washing: 10 mM potassium phosphate, pH 3
elution: 1 mL 60% methanol in DI water.
column: Discovery C18, 15 cm x 4.6 mm I.D., 5 μm particles (504955)
mobile phase: [A] 10 mM KH2PO4, pH 3 (adjusted with H3PO4): [B] acetonitrile; (60:40, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: UV, 234 nm
injection: 10 μL
suitability
application for HPLC
application for SPE
application(s)
forensics and toxicology
Application
Neat horse serum was acidified with 10 mL 6 M HCl/mL serum. Internal standard (I.S.) blank was prepared by spiking acidified horse serum with indapamide at 10 mg/mL. Sample A = 10 mg/mL furosemide in I.S. blank. Sample B = 5 mg/mL furosemide in I.S. blank. Sample C = 0.5 mg/mL furosemide in I.S. blank. Sample D = 0.1 mg/mL furosemide in I.S. blank. Sample E = 0.05 mg/mL furosemide in I.S. blank.
Legal Information
SPE tube or plate
analytical column
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service