G000595
HPLC Analysis of Tricyclic Antidepressants in Serum on Discovery® C18 after SPE using Discovery® DSC-18
application for SPE, application for HPLC
About This Item
technique(s)
HPLC: suitable
solid phase extraction (SPE): suitable
test parameters
sample preparation: SPE (Solid Phase Extraction)
SPE tube/cartridge: Discovery DSC-18, 100 mg/1 mL (52602-U)
column: Discovery C18, 15 cm × 4.6 mm, 5 μm preceded by a 2 cm C18 guard column and 0.5 μm frit filter (504955)
mobile phase: [A] acetonitrile: [B] methanol: [C] 25 mM potassium phosphate, monobasic (pH 7 with triethlylamine) (45:25:30, A:B:C)
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 254 nm
injection: 50 μL, diluted porcine serum extract
suitability
application for HPLC
application for SPE
application(s)
clinical
Legal Information
Analyte
analytical column
related product
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