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845125

TOYOPEARL® Butyl-650M MiniChrom Column

phase C4 (butyl), 65 μm particle size, pore size 1000 Å, L × I.D. 10 cm × 8 mm, MiniChrom Column

Synonym(s):

MiniChrom

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About This Item

UNSPSC Code:
41115700

material

PEEK

product line

TOYOPEARL®

analyte chemical class(es)

proteins

manufacturer/tradename

Tosoh 45125

parameter

3 bar max. pressure (resin)
30 bar max. pressure (column)

technique(s)

HPLC: suitable
LPLC: suitable

L × I.D.

10 cm × 8 mm

bed volume

5 mL

matrix

hydroxylated methacrylic polymer

matrix active group

C4 (butyl) phase

particle size

65 μm

pore size

1000 Å

operating pH

1-13

capacity

40 g/L±10 adsorption capacity (lysozyme)

separation technique

hydrophobic interaction (HIC)

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General description

MiniChrom columns for TOYOPEARL® and TSKgel® are pre-packed with the most popular ion exchange, mixed-mode, hydrophobic interaction or affinity media. These columns are available in 5 mL volume, 8 mm ID x 10 cm L, and can be operated with standard laboratory LC systems. MiniChrom columns for TOYOPEARL and TSKgel provide:
  • Direct connection to common laboratory LC Systems
  • Easy parameter screening and method optimization
  • Fast small scale purification
  • Reliable and reproducible results

Compatibility

Columns are tolerant to aqueous buffers and salt solutions, 1 mol/L alkaline solutions, 1 mol/L non-oxidizing mineral acids, 8 mol/L urea, 6 mol/L guanidine hydrochloride, non-halogenated organic solvents and detergents. They are not compatible with strong oxidants and halogenated solvents.

Specifications

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physical form

Shipped in 20% (v/v) ethanol, 0.15 M NaCl.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation
Toyopearl is a registered trademark of Tosoh Corporation

Regulatory Information

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