84-109
HPLC Analysis of Triazine Herbicides in Grass on SUPELCOSIL™ LC-8-DB after SPE using Supelclean™ LC-SCX
application for SPE, application for HPLC
About This Item
technique(s)
HPLC: suitable
solid phase extraction (SPE): suitable
test parameters
sample preparation: SPE (Solid Phase Extraction)
sample/matrix: fresh grass clippings spiked with herbicides at 2 ppm
SPE tube/cartridge: Supelclean LC-SCX, 500 mg/3 mL (57018)
condition: 1 mL methylene chloride
sample addition: 2 mL grass extract. Wash with 2 x 2 mL acetonitrile.
drying: Dry packing for 5 min. under nitrogen purge
washing: Dry packing for 5 min under nitrogen purge; wash with 2 x 2 mL DI water
elution: 1.5 mL methanol
eluate post-treatment: dilute to 2 mL with DI water
column: SUPELCOSIL LC-8-DB, 15 cm × 4.6 mm I.D., 5 μm (58347-U) with guard column (59553)
mobile phase: [A] acetonitrile; [B] water; (45:55, A:B)
flow rate: 1.5 mL/min
detector: UV, 254 nm
injection: 100 μL
suitability
application for HPLC
application for SPE
application(s)
environmental
Application
5 g fresh grass clippings spiked with 2 ppm each herbicide. Add 4 g anhydrous sodium sulfate and 20 mL methylene chloride:acetone (80:20). Shake 20 min and allow mixture to stand 1 min.
Legal Information
Analyte
SPE tube or plate
analytical column
guard column
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