823426
TOYOPEARL® AF-rProtein A HC-650F Bulk Media
phase rProtein A, bottle of 25 mL, 45 μm particle size
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description
Adsorption capacity (> 80 g/L IgG (static); >70 g/L IgG (dynamic at 5 min. residence time))
Phase filter (Protein A)
product line
TOYOPEARL®
form
slurry
packaging
bottle of 25 mL
manufacturer/tradename
Tosoh 23426
parameter
3 bar max. pressure
technique(s)
LPLC: suitable
matrix
hydroxylated methacrylic polymer particle platform
matrix active group
rProtein A phase
particle size
45 μm
pore size
1000 Å
cleaning in place
0.1 M NaOH
pH
2-13
capacity
>70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time)
>80 g/L adsorption capacity (IgG)(static)
separation technique
affinity
storage temp.
2-8°C
General description
TOYOPEARL AF-rProtein A HC-650F resin maintains 80% of initial dynamic binding capacity after 40 CIP cycles with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A HC-650F resin exhibits dynamic binding capacities of greater than 65 g/L at residence times of 5 minutes and greater than 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L.
Achievement of high linear velocities at relatively low pressure enables high throughput at production scale using equipment with moderate pressure limitations. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to agarose base stable resins. Typical leakage for this rProtein A ligand is 0.6 -1.7 ng rProtein A /mg eluted antibody by ELISA testing. Though TOYOPEARL AF-rProtein A-650F resin is shipped in 20% ethanol, it can be stored in 2% benzyl alcohol if necessary.
Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at low residence times.
Specifications
Physical form
Legal Information
related product
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
WGK
WGK 3
Flash Point(F)
104.0 °F
Flash Point(C)
40 °C
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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