822856
TSKgel® UltraSW Aggregate (3 µm) HPLC Columns
L x I.D. 30 cm × 7.8 mm, diol phase
Synonym(s):
TSKgel® Size Exclusion (SW-Type) HPLC Column
About This Item
product name
TSKgel® UltraSW Aggregate HPLC Column, phase diol, L × I.D. 30 cm × 7.8 mm, 3 μm particle size
material
stainless steel
Agency
suitable for USP L20
suitable for USP L59
product line
TSKgel®
manufacturer/tradename
Tosoh UltraSW Aggregate, 22856
parameter
≤0.5 M salt concentration
0-100% aqueous soluble organic solvents
1 mL/min flow rate
10-30 °C temperature
120 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
30 cm × 7.8 mm
matrix
silica particle platform
matrix active group
diol phase
particle size
3 μm
pore size
300 Å
operating pH
2.5-7.5
separation technique
size exclusion (SEC)
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General description
TSKgel UltraSW Aggregate columns have a slightly larger pore size and smaller (3 μm) particle size than the industry-leading TSKgel G3000SWxl (5 μm) and SuperSW3000 (4 μm) columns. These 30 cm x 7.8 mm ID silica-based, stainless steel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates.
The calibration curve of the TSKgel UltraSW Aggregrate column shows a separation range up to around 2 million Da, which provides better resolution of aggregates/multimers of a mAb than the TSKgel SuperSW mAb columns..
Physical form
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Articles
Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.
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