821982
TSKgel® Amide-80 (5 µm) HPLC Columns
L x I.D. 25 cm × 4.6 mm, carbamoyl phase
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product name
TSKgel® Amide-80 HPLC Column, phase carbamoyl, L × I.D. 25 cm × 4.6 mm, 5 μm particle size
material
stainless steel hardware
Agency
suitable for USP L68
description
Amide-80 HR
product line
TSKgel®
manufacturer/tradename
Tosoh 21982
parameter
0-100% organic solvent
10-80 °C temperature
150 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface area
450 m2/g
matrix
silica particle platform
matrix active group
carbamoyl phase
particle size
5 μm
pore size
100 Å
operating pH
2.0-7.5
separation technique
hydrophilic interaction (HILIC)
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General description
Packed with 2, 3, 5, or 10 μm spherical silica particles that are covalently bonded with carbamoyl groups, TSKgel Amide-80 columns are used for the analysis of saccharides, glycans, oligosaccharides, peptides and polar compounds from natural products. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography.
TSKgel Amide-80 HR, 5 μm columns offer higher specifications for plate count compared to traditional TSKgel Amide-80, 5 μm, 4.6 mm ID x 25 cm columns (N = > 18,000 vs. 8,000). This equates to more efficient chromatography.
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