813066
TSKgel® Boronate-5PW (10 µm) HPLC Columns
L x I.D. 7.5 cm × 7.5 mm, m-aminophenyl boronate phase
product name
TSKgel® Boronate-5PW HPLC Column, phase m-aminophenyl boronate, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size
material
PEEK
product line
TSKgel®
analyte chemical class(es)
proteins
manufacturer/tradename
Tosoh Boronate-5PW, 13066
parameter
≤20% organic solvent
≤3 M salt concentration
1.2 mL/min flow rate
30 bar max. pressure
≥ 0 °C temperature
technique(s)
HPLC: suitable
L × I.D.
7.5 cm × 7.5 mm
matrix
polymer particle platform
matrix active group
m-aminophenyl boronate phase
particle size
10 μm
pore size
1000 Å
operating pH
2-9
capacity
40 μmol/mL adsorption capacity (sorbitol)
compatibility
mode of use affinity HPLC
separation technique
affinity
storage temp.
2-8°C
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General description
Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.
Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.
Legal Information
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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