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TOYOPEARL® DEAE-650C Bulk Media

phase DEAE (diethylaminoethyl), bottle of 250 mL, 100 μm particle size

Synonym(s):

TOYOPEARL® DEAE-650C Bulk Media

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About This Item

UNSPSC Code:
47101511

description

DEAE-650C

product line

TOYOPEARL®

form

slurry

feature

counter ion Cl-

packaging

bottle of 250 mL

parameter

3 bar max. pressure

technique(s)

HPLC: suitable

matrix

methacrylate

matrix active group

DEAE (diethylaminoethyl) phase

particle size

100 μm

operating pH

2-10

capacity

0.10 meq/mL±0.02 meq/mL ion exchange capacity
30 g/L±5 adsorption capacity (BSA)
30 mg/mL±5 mg/mL binding capacity (bovine serum albumin)

compatibility

mode of use weak anion exchange chromatography

separation technique

anion exchange

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General description

Toyopearl DEAE-650 resin is a weak anion exchanger for blood fractionation and other biomolecule purification. It can be used for high throughput capture, intermediate purification, and polishing process steps. If more powerful resolution is needed TSKgel DEAE-5PW resin should be used. Selectivity remains the same.

Application

TOYOPEARL® DEAE-650C is used in separation media and ion exchange resins. TOYOPEARL® DEAE-650C has been used in studies to develop supplements for immune enhancement.

Specifications

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physical form

Shipped in 20% (v/v) ethanol.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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H Lee et al.
Applied biochemistry and biotechnology, 97(2), 123-133 (2002-05-09)
A keratinolytic protease-producing microorganism was isolated from soybean paste waste and was identified as a strain of Bacillus sp. The keratinase was purified by polyethylene glycol precipitation and two successive column chromatographies with DEAE-Toyopearl 650C and Sephacryl S-200 HR. The
Hyug Chun et al.
Biological & pharmaceutical bulletin, 25(9), 1203-1208 (2002-09-17)
The purified polysaccharides from Piper nigrum were prepared as follows: a hot water extract of pepper seeds was fractionated by ultrafiltration with a 5-kDa-membrane cartridge. A fraction with 5 kDa or bigger molecules was successively purified by open column chromatography
H Chun et al.
Biological & pharmaceutical bulletin, 24(8), 941-946 (2001-08-21)
Polysaccharides are involved in biological responses and can activate complement system, which plays an important role in primary host defense mechanisms. We investigated anticomplementary activities from spice plants and selected thyme (Thymus vulgaris L.) as a potent complementary activator. Acidic

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