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TOYOPEARL® CM-650M Bulk Media

phase carboxymethyl, bottle of 250 mL, 65 μm particle size

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Synonym(s):
TOYOPEARL® CM-650 Bulk Media
UNSPSC Code:
47101511

description

CM-650M

product line

TOYOPEARL®

form

slurry

feature

counter ion Na+

packaging

bottle of 250 mL

parameter

3 bar max. pressure

technique(s)

HPLC: suitable

matrix

methacrylate

matrix active group

carboxymethyl phase

particle size

65 μm

operating pH

5-10

capacity

0.10 meq/mL±0.02 meq/mL ion exchange capacity
 40 g/L±10 adsorption capacity (lysozyme)
 50 mg/mL±10 mg/mL binding capacity (bovine haemoglobin)

compatibility

mode of use weak cation exchange chromatography

separation technique

cation exchange

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General description

Toyopearl CM-650 resin is a weak cation exchanger for biomolecule purification. It can be used for high throughput capture, intermediate purification, and polishing process steps.

Application

TOYOPEARL® CM-650M is used in separation media and ion exchange resins. TOYOPEARL® CM-650M has been used in cloning studies.

Specifications

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physical form

Shipped in 20% (v/v) ethanol.

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Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shuuji Ootsuka et al.
Journal of biotechnology, 125(2), 269-280 (2006-04-20)
An endo-beta-1,4-mannanase was isolated from digestive fluid of Pacific abalone, Haliotis discus hannai, by successive chromatographies on TOYPEARL CM-650M, hydroxyapatite, and TOYOPEARL HW50F. The abalone mannanase, named HdMan in the present paper, showed a molecular mass of approximately 39,000 Da
Ken-ichi Suzuki et al.
European journal of biochemistry, 270(4), 771-778 (2003-02-13)
A cellulase [endo-beta-1,4-D-glucanase (EC 3.2.1.4)] was isolated from the hepatopancreas of abalone Haliotis discus hannai by successive chromatographies on TOYOPEARL CM-650M, hydroxyapatite and Sephacryl S-200 HR. The molecular mass of the cellulase was estimated to be 66 000 Da by
Eri Shimizu et al.
Carbohydrate research, 338(24), 2841-2852 (2003-12-12)
An alginate lyase, termed HdAly in the present paper, was isolated from the hepatopancreas of abalone, Haliotis discus hannai, by ammonium sulfate fractionation, followed by TOYOPEARL CM-650M column chromatography. Enzymatic properties of HdAly were similar to those of previously reported

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