807469
TOYOPEARL® HW-75F Bulk Media
phase hydroxylated methacrylate, bottle of 500 mL, 45 μm particle size
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Synonym(s):
TOYOPEARL® HW-75 Size Exclusion Media
UNSPSC Code:
41115700
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description
HW-75F
product line
TOYOPEARL®
form
slurry
packaging
bottle of 500 mL
parameter
3 bar max. pressure
technique(s)
HPLC: suitable
matrix
hydroxylated methacrylic polymer
matrix active group
hydroxylated methacrylate phase
particle size
45 μm
pore size
100,000-10,000,000 Da MW range
operating pH
2-13
separation technique
size exclusion (SEC)
General description
Toyopearl HW-75 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 500,000-50,000,000 Da.
Application
TOYOPEARL® media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.
Specifications
Clean in place with 0.5 M NaOH or 0.1 M HCl.
Physical form
Shipped in 20% (v/v) ethanol.
Legal Information
Toyopearl is a registered trademark of Tosoh Corporation
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
WGK
WGK 1
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Shu-fen Xu et al.
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin
M V Pokrovskaya et al.
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We
Olga N Smol'kina et al.
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was
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