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805104

TSKgel® G4000SW (13 µm) HPLC Columns

L x I.D. 60 cm × 7.5 mm, diol phase

Synonym(s):

TSKgel® Size Exclusion (SW-Type) HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

product name

TSKgel® G4000SW HPLC Column, phase diol, L × I.D. 60 cm × 7.5 mm, 13 μm particle size

material

stainless steel

Agency

suitable for USP L59

product line

TSKgel®

manufacturer/tradename

Tosoh 05104

parameter

≤0.5 M salt concentration
0-100% aqueous soluble organic solvents
1.2 mL/min flow rate
10-30 °C temperature
30 bar max. pressure

technique(s)

HPLC: suitable

L × I.D.

60 cm × 7.5 mm

matrix

silica particle platform

matrix active group

diol phase

particle size

13 μm

pore size

450 Å

operating pH

2.5-7.5

separation technique

size exclusion (SEC)

General description

As the first generation of silicia based SEC columns, the TSKgel G4000SW columns provide excellent separation of proteins and other biomolecules. TSKgel G4000SW columns are available in 13μm particle size in Glass and Stainless Steel analytical columns. Particle sizes of 13 and 17μm are used to pack 30cm and 60cm x 21.5mm ID columns, respectively, for semi-preparative purifications.

Physical form

Shipped in 0.05% NaN3 and 0.1 mol/L Na2SO4 in 0.1 M phosphate buffer, pH 6.7.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the pressure rating for the 60cm TSK-GEL G4000SW, Product 805104?

    The TSKgel G4000SW, 60cm x 7.5mm (Product No. 805104) is rated to a maximum pressure of 450 psi; this converts to 30 kg/cm2. The 30cm column (Product No. 805790) is rated to 225 psi (15 kg/cm2).

  4. How does Product 805104, TSKgel® G4000SW compare to TSKgel®G4000SWXL?

    The TSKgel G4000SW columns are packed with a 13 micron spherical silica particle; the TSKgel G4000SWXL is packed with an 8 micron particle.  For practical use, the G4000SW gives half the resolution, and requires a larger sample size, so it is recommended when sample quantities are not limited.

  5. What is the separation range for the TSK-GEL G4000 SWXL, Product 805104?

    The separation ranges for TSK-GEL G4000 SWXL packing are: Globular protein 2 x 104 - 1 x 107 Da, Dextrans 4 x 103 - 5 x 105 Da, Polyethylene glycol & oxides 2 x 103 - 2.5 x 105 Da.  The pore size is listed as 450 Å.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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