HPLC Analysis of Paraquat and Diquat on SUPELCOSIL^™ LC-18 after SPE using ENVI^™-8 DSK

technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: drinking water adjusted to pH to 10.
sample preparation: ENVI-8 DSK, 47 mm (57172)
SPE tube/cartridge: ENVI-8 DSK SPE Disk, 47 mm (57172)
condition: 10 mL acetonitrile; 2 × 10 mL reagent water; 10 mL conditioning solvent A (5 g cetyl trimethyl ammonium bromide and 5 mL conc. ammonium hydroxide in 500 mL DI water, dilute to 1 L); (2 × 10 mL reagent water; 10 mL conditioning solvent B (10 g hexanesulfonic acid, sodium salt and 10 mL conc. ammonium hydroxide in 250 mL DI water, dilute to 500 mL))
sample addition: 250 mL water 100 mL/min
elution: 0.5-1.0 mL acetonitrile (to cover/solvate disk); 2 × 4 mL eluting solution (13.5 mL orthophosphoric acid and 10.3 mL diethylamine in 500 mL DI water, dilute to 1 L)
column: SUPELCOSIL LC-18, 15 cm × 4.6 mm I.D., 5 μm particles (58230-U)
mobile phase: 3.5 mL triethylamine and 1.0 g 1-hexane-sulfonic acid, sodium salt to 800 mL DI H₂O add orthophosphoric acid to pH 2.5, dilute to 1 L
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: photodiode array, diquat - 308 nm, paraquat - 257 nm
injection: 100 μL

suitability

application for HPLC
application for SPE

application(s)

environmental