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BIOshell A160 Peptide CN (5 μm) HPLC Columns

L × I.D. 5 cm × 75 μm, HPLC Column

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UNSPSC Code:
41115700
NACRES:
SB.52

product name

BIOshell A160 Peptide CN, 5 μm HPLC Column, 5 μm particle size, L × I.D. 5 cm × 75 μm

material

stainless steel hardware (column)

Agency

suitable for USP L10

description

Shell thickness (0.6 μm)
Solid Core (3.5 μm)

product line

BIOshell

feature

endcapped

analyte chemical class(es)

peptides

manufacturer/tradename

BIOshell

packaging

1 ea of

extent of labeling

1.5% carbon loading

parameter

600 bar max. pressure
90 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

5 cm × 75 μm

surface area

60 m2/g

surface coverage

2.2 μmol/m2

matrix

spherical silica particle platform
superficially porous particle

matrix active group

cyano phase

particle size

5 μm

pore size

160 Å

operating pH range

1-8

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

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General description

BIOshell 160 Å Peptide CN columns provide an alternative selectivity to the traditional C18 bonded phases. The introduction of the cyano group enables peptides and small proteins to be separated due to a combination of hydrophobicity and dipole-dipole interactions making this column an excellent choice for biomolecules that are not resolved on traditional C18 columns. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected cyanopropyl ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C).

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