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BIOshell A160 Peptide C18 (2.7 μm) HPLC Columns

L × I.D. 15 cm × 1 mm, HPLC Column

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UNSPSC Code:
41115700
NACRES:
SB.52

product name

BIOshell A160 Peptide C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 1 mm

material

stainless steel hardware

Quality Level

Agency

suitable for USP L1

description

Shell thickness (0.5 μm)
Solid Core (1.7 μm)

product line

BIOshell
BIOshell

feature

endcapped: no

analyte chemical class(es)

peptides
peptides

manufacturer/tradename

BIOshell
BIOshell

packaging

1 ea of

extent of labeling

4.6% carbon loading

parameter

600 bar max. pressure
90 °C max. temp.

technique(s)

HPLC: suitable
HPLC: suitable
LC/MS: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
UHPLC: suitable

L × I.D.

15 cm × 1 mm

surface area

90 m2/g

surface coverage

2.2 μmol/m2

matrix

spherical silica particle platform
superficially porous particle
superficially porous particle

matrix active group

C18 (octadecyl) bonding phase, diisobutyloctadecyl

particle size

2.7 μm
2.7 μm

pore size

160 Å

operating pH range

1-8

separation technique

reversed phase
reversed phase

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General description

BIOshell 160 Å Peptide C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected C18 ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C). This greatly expands the application range for the separation of biomolecules.

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Articles

Standards are critical in liquid chromatography/mass spectrometry (LC/MS) based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis.

An optimized peptide mapping protocol using NISTmAb as a model monoclonal antibody, shorter incubation times, and improved digestion buffer to demonstrate minimal artificial asparagine deamidation and methionine oxidation.

Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results and high efficiency.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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