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BIOshell A160 Peptide C18 (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

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Synonym(s):
Superficially Porous Wide Pore C18 UHPLC Column
UNSPSC Code:
41115700
NACRES:
SB.52

product name

BIOshell A160 Peptide C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

material

stainless steel hardware

Quality Level

100

Agency

suitable for USP L1

description

Shell thickness (0.5 μm)
Solid Core (1.7 μm)

product line

BIOshell
BIOshell

feature

endcapped: no

analyte chemical class(es)

peptides
peptides

manufacturer/tradename

BIOshell
BIOshell

packaging

1 ea of

extent of labeling

4.6% carbon loading

parameter

600 bar max. pressure
90 °C max. temp.

technique(s)

HPLC: suitable
HPLC: suitable
LC/MS: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
UHPLC: suitable

L × I.D.

15 cm × 2.1 mm

surface area

90 m2/g

surface coverage

2.2 μmol/m2

matrix

spherical silica particle platform
superficially porous particle
superficially porous particle

matrix active group

C18 (octadecyl) bonding phase, diisobutyloctadecyl

particle size

2.7 μm
2.7 μm

pore size

160 Å

operating pH range

1-8

separation technique

reversed phase
reversed phase

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General description

BIOshell 160 Å Peptide C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected C18 ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C). This greatly expands the application range for the separation of biomolecules.

Application

UHPLC Analysis of Peptides on BIOshell A160 Peptide C18, CN, and Phenyl-Hexyl, 2.7 μm
BIOshell A160 Peptide C18, 2.7 μm HPLC Column, a non-end capped column that has superficially porous particle with C18 alkyl ligand as the stationary phase, is used mainly in the separation of peptides at a good flow rate.

Features and Benefits

BIOshell A160 Peptide C18, 2.7 μm HPLC Columns are compatible both with UHPLC and HPLC systems. They are specifically engineered for the fast and high-resolution seperation of biomolecules. These columns operate at high flow rates and have 40% higher efficiency than columns with fully porous particles of the same size. They are stable at high temperatures, which increases the throughput and enhances the peak shape.

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Collagen-derived X-Hyp-Gly-type tripeptides promote differentiation of MC3T3-E1 pre-osteoblasts
Taga Y, et al.
Journal of functional foods, 46, 456-462 (2018)
Jennifer K Field et al.
Journal of chromatography. A, 1622, 461093-461093 (2020-04-29)
The Peptide RPC Column Characterisation Protocol was applied to 38 stationary phases, varying in ligand chemistry, base silica, end capping and pore size, which are suitable for the analysis of peptides. The protocol at low and intermediate pH is based

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