59132
SUPELCOSIL™ LC-NH₂ NP (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
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Synonym(s):
SUPELCOSIL™ LC-NH2-NP 5UM 25CMX4.6MM HPLC COLUMN
UNSPSC Code:
41115700
eCl@ss:
32110501
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product name
SUPELCOSIL™ LC-NH2-NP HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Agency
suitable for USP L8
Quality Level
feature
endcapped
manufacturer/tradename
SUPELCOSIL™
extent of labeling
3% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface area
170 m2/g
surface coverage
surface coverage 5.1 μmol/m2
matrix
silica gel, spherical particle platform
matrix active group
amino phase
particle size
5 μm
pore size
120 Å
pH
2-7.5
application(s)
food and beverages
separation technique
normal phase
Related Categories
General description
An amino phase dedicated to normal-phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal-phase chromatography. Normal-phase chromatography is especially useful when the analytes are not water soluble -- for example, the fat-soluble vitamins A, D, E, and K.
These columns should be used with non-aqueous mobile phases only.
These columns should be used with non-aqueous mobile phases only.
SUPELCOSIL LC-NH2-NP is an amino phase dedicated to normal phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal phase chromatography.
Features and Benefits
• show stable retention in normal-phase separations
• are less sensitive to small or varying amounts of water in mobile phases, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins
• are less sensitive to small or varying amounts of water in mobile phases, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins
SUPELCOSIL LC-NH2-NP columns:
• show stable retention in normal phase separations
• are less sensitive to small or varying amounts of water in the mobile phase, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins
• show stable retention in normal phase separations
• are less sensitive to small or varying amounts of water in the mobile phase, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Regulatory Information
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E M Koves
Journal of chromatography. A, 692(1-2), 103-119 (1995-02-10)
A comprehensive approach to the analysis for many drugs in postmortem blood and biological fluids using high-performance liquid chromatography and diode array detection has been developed. To reduce the likelihood of co-eluting interference components of postmortem blood or other drugs
S Djabarouti et al.
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A simple and sensitive high-performance liquid chromatography (HPLC) method has been developed and validated for the determination of DRF-1042, a novel orally active camptothecin (CPT) analog, in human plasma. The sample preparation was a simple deproteinization with acidified methanol yielding
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A sensitive, simple, and reliable high-performance liquid chromatographic method with electrochemical detection is developed for the measurement of four natural products, the serotonin-related indols from human platelet-rich plasma (PRP) using N-methylserotonin as internal standard. Separation of serotonin (5HT), 5-hydroxytryptophan (5HTP)
K K Peh et al.
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