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58201

Supelco

SUPELCOSIL™ LC-Diol (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

SUPELCOSIL™ LC-DIOL 5UM 25CMX4.6MM HPLC COLUMN

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

Product Name

SUPELCOSIL™ LC-Diol HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Agency

suitable for USP L20

Quality Level

100

feature

endcapped: no

packaging

pkg of 1 ea

manufacturer/tradename

SUPELCOSIL™

extent of labeling

3.5% carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 3.8 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

diol phase

particle size

5 μm

pore size

120 Å pore size

pH range

2-7.5

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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General description

LC-Diol columns can be used to separate proteins by gel filtration chromatography. When operated with an aqueous buffer, the diol phase can effectively shield the silica surface from interacting with proteins. A well-known use of diol columns, under normal phase conditions, is the separation of steroids and sterols.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H371

Hazard Classifications

STOT SE 2 Inhalation

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
293852-04
293852-03
293852-02
293852-01
293788-04

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the phase for Supelcosil LC-Diol HPLC column, Product 58201?

    The SUPELCOSIL LC-DIOL phase is a glycidoxypropylsilyl.

  4. Can Supelcosil LC-Diol HPLC column, Product 58201, be use with acid?

    No. The SUPELCOSIL LC-DIOL is very susceptible to cleaving of the phase using acids, therefore we do not recommend using acids, not even 1% acetic acid.

  5. Can Supelcosil LC-Diol HPLC column, Product 58201, be used in a reversed-phase and normal phase system?

    Yes. Before moving from one mode to another, the column should be flushed thoroughly with IPA (isopropyl alcohol).

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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