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Supelco

Supelclean™ LC-18 SPE Tube

bed wt. 500 mg, volume 6 mL, pkg of 30 ea

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UNSPSC Code:
41115712

material

PE frit (20 μm porosity)
polypropylene hardware

Quality Level

100

product line

Supelclean™

feature

endcapped

composition

bed wt., 500 mg

packaging

pkg of 30 ea

extent of labeling

~11.5% C loading

technique(s)

solid phase extraction (SPE): suitable

surface area

475 m2/g

volume

6 mL

matrix

silica gel base material (irregularly shaped)

matrix active group

C18 (octadecyl) bonding, monomerically bonded

particle size

45 μm

pore size

0.8 cm3/g pore volume
60 Å pore size

application(s)

food and beverages

separation technique

reversed phase

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General description

Solid phase extraction apparatus uses glass vials or tubes for sample collection and concentration. Some sorbents require large desorption volume than small SPE cartridges in such case SPE tubes were used. The 6 mL barrel is compatible with most vacuum-manifold devices and allows accurate loading of samples.

Application

Supelclean™ LC-18 SPE Tube was used in SPE to purify primary prostaglandins PGE2, PGF2α and their tissue metabolites.

Legal Information

Supelclean is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Toni N Welsh et al.
Prostaglandins & other lipid mediators, 83(4), 304-310 (2007-05-15)
The primary prostaglandins PGE(2) and PGF(2 alpha) are metabolized in tissues by a series of enzymatic and non-enzymatic reactions. To measure metabolic rates and individual reaction rates it is necessary to extract the parent prostaglandins and metabolites before the separation
Nigel J.K. Simpson
Solid-Phase Extraction: Principles, Techniques, and Applications, 445-445 (2010)

Articles

Solid Phase Extraction: Reversed-Phase Methodology

Reversed-phase interaction will retain most molecules with hydrophobic character; it is very useful for extracting analytes that are very diverse in structure within the same sample.

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