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Discovery® BIO Wide Pore C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4 mm

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UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Discovery® BIO Wide Pore C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L1

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

9.2% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

25 cm × 4 mm

surface area

100 m2/g

surface coverage

3.6 μmol/m2

matrix

silica particle platform
fully porous particle

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

300 Å

operating pH

2-8

separation technique

reversed phase

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General description

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Regulatory Information

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DiscoveryBIO Wide Pore C18

DiscoveryBIO Wide Pore C18 products offered by Sigma-Aldrich.

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