Skip to Content

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

Merck
CN
All Photos(1)

Key Documents

567536-U

Supelco

Discovery® HS F5 (5 µm) HPLC Columns

L × I.D. 25 cm × 4 mm, HPLC Column

Synonym(s):

Discovery Pentafluorophenyl PFP HPLC Column

Sign Into View Organizational & Contract Pricing

Select a Size

1 EA
CN¥9,028.17

CN¥9,028.17

Available to ship onApril 14, 2025Details

All Photos(1)

Select a Size

Change View
1 EA
CN¥9,028.17

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

CN¥9,028.17

Available to ship onApril 14, 2025Details

Product Name

Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm

material

stainless steel column

Agency

suitable for USP L43

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

12% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
567531U567530U567501U
Discovery® HS F5 HPLC Column 5 μm particle size, L × I.D. 25 cm × 4 mm

567536U

Discovery® HS F5 HPLC Column

Discovery® HS F5 HPLC Column 3 μm particle size, L × I.D. 10 cm × 4 mm

567531U

Discovery® HS F5 HPLC Column

Discovery® HS F5 HPLC Column 3 μm particle size, L × I.D. 5 cm × 4 mm

567530U

Discovery® HS F5 HPLC Column

Discovery® HS F5 HPLC Column 3 μm particle size, L × I.D. 3.3 cm × 2.1 mm

567501U

Discovery® HS F5 HPLC Column

matrix active group

PFP (pentafluorophenyl) phase

matrix active group

PFP (pentafluorophenyl) phase

matrix active group

PFP (pentafluorophenyl) phase

matrix active group

PFP (pentafluorophenyl) phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

matrix

silica gel, high purity, spherical particle platform, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

pore size

120 Å

pore size

120 Å

pore size

120 Å

pore size

120 Å

General description

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Features and Benefits

  • Unique selectivity
  • Similar retention to C18 (sometimes requires stronger mobile phase)
  • Excellent peak shape
  • Stable, low-bleed LC-MS separations
  • Scalable separations from 3 to 10μm particle sizes

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

guard cartridge

Product No.
Description
Pricing

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
292504-01
292523-01
292434-01
292433-01
292432-01

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Jennie Wang et al.
Journal of pharmaceutical and biomedical analysis, 124, 267-273 (2016-03-13)
An HPLC method for the assay of a DNA topoisomerase inhibitor, LMP776 (NSC 725776), has been developed and validated. The stress testing of LMP776 was carried out in accordance with International Conference on Harmonization (ICH) guidelines Q1A (R2) under acidic
Umberto M Battisti et al.
Journal of chromatography. A, 1443, 152-161 (2016-03-30)
A "heart-cut" two-dimensional achiral-chiral liquid chromatography triple-quadrupole mass spectrometry method (LC-LC-MS/MS) was developed and coupled to in vivo cerebral microdialysis to evaluate the brain response to the chiral compound (±)-7-chloro-5-(3-furanyl)-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine-1,1-dioxide ((±)-1), a potent positive allosteric modulator (PAM) of AMPA receptor.
Jana Maláková et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 853(1-2), 265-274 (2007-04-10)
Galantamine, an alkaloid isolated from the bulbs and flowers of Caucasian snowdrop (Galanthus woronowii, Amaryllidaceae) and related species, is employed in human medicine for the treatment of various neuromuscular and neurodegenerative diseases. After the administration, the products of oxidative biotransformation
Lucie Havlíková et al.
Talanta, 76(3), 597-601 (2008-07-01)
A pentafluorophenylpropyl (PFPP) stationary phase was for the first time tested for the simultaneous determination of triamcinolone acetonide, its degradation product triamcinolone and two preservatives, methylparaben, and propylparaben. A new simple isocratic reversed phase HPLC method with UV detection, using
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)
In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service