567520-U
Discovery® HS F5 (5 µm) HPLC Columns
L × I.D. 25 cm × 10 mm, HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Pricing and availability is not currently available.
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Product Name
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
material
stainless steel column
Agency
suitable for USP L43
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
12% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
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Related Categories
1 of 4
This Item | 07102 | 320331 | 258148 |
|---|---|---|---|
| assay ≥37% | assay 36.5-38% | assay 36.5-38.0% (ACS specification), 37% | assay 36.5-38.0% (ACS specification), 37% |
| agency USP/NF, reag. Ph. Eur., reag. ISO | agency - | agency suitable for GB 5009.253-2016, suitable for SM 5210 | agency suitable for GB 5009.253-2016, suitable for SM 5210 |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form liquid | form liquid |
| bp >100 °C (lit.) | bp >100 °C (lit.) | bp >100 °C (lit.) | bp >100 °C (lit.) |
General description
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Features and Benefits
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Regulatory Information
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Lucie Havlíková et al.
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A pentafluorophenylpropyl (PFPP) stationary phase was for the first time tested for the simultaneous determination of triamcinolone acetonide, its degradation product triamcinolone and two preservatives, methylparaben, and propylparaben. A new simple isocratic reversed phase HPLC method with UV detection, using
Federica Pellati et al.
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In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
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