Skip to Content
Merck
CN
All Photos(1)

Documents

567209-U

Supelco

Discovery® BIO Wide Pore C18 (10 µm) HPLC Columns

L × I.D. 25 cm × 10 mm

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Discovery® BIO Wide Pore C18 HPLC Column, 10 μm particle size, L × I.D. 25 cm × 10 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L1

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

9.2% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

25 cm × 10 mm

surface area

100 m2/g

surface coverage

3.6 μmol/m2

matrix

silica particle platform
fully porous particle

matrix active group

C18 (octadecyl) phase

particle size

10 μm

pore size

300 Å

operating pH

2-8

separation technique

reversed phase

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
289898-01
289833-01
289832-01
287916-01
287640-01

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

DiscoveryBIO Wide Pore C18

DiscoveryBIO Wide Pore C18 products offered by Sigma-Aldrich.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service