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Merck
CN

567202-U

Discovery® BIO Wide Pore C18 (3 µm) HPLC Columns

L × I.D. 15 cm × 2.1 mm

Synonym(s):

Wide Pore Biological C18 Chromatography Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

Key Documents

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Product Name

Discovery® BIO Wide Pore C18 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 2.1 mm

Quality Level

100

material

stainless steel column

agency

suitable for USP L1

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

9.2% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 2.1 mm

surface area

100 m2/g

surface coverage

3.6 μmol/m2

matrix

silica particle platform
fully porous particle

matrix active group

C18 (octadecyl) phase

particle size

3 μm

pore size

300 Å

operating pH

2-8

separation technique

reversed phase

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General description

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificates of Analysis (COA)

Lot/Batch Number
299865-06
299865-05
299865-04
299865-03
299865-02

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Large Molecule HPLC

High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.

大分子HPLC_生物分子反相HPLC_高效液相色谱法-默克生命科学

生物分子是活细胞产生的大分子聚合物，作为活生物体的基本组成部分执行许多生命必需的生物过程。尽管现有很多分离和分析技术可供选择，最常用的还是高效液相色谱法(HPLC)。

Large Molecule HPLC

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

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