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Supelco

apHera™ NH₂ (5 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

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Synonym(s):
Amino HPLC Column
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

apHera™ NH2 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L82

product line

apHera™

packaging

pkg of 1 ea

manufacturer/tradename

apHera™

parameter

147 bar pressure (2133 psi)
4-50 °C temperature

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

matrix

polymeric

matrix active group

amino phase

particle size

5 μm

pore size

300 Å

operating pH

1-14

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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General description

apHera™ amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column.

Features of Amino apHera™ Columns
  • High efficiency for carbohydrate analysis
  • Ideal for basic conditions/amphoteric detection
  • High selectivity mono- to oligosaccharides
  • pH range 2-13
  • 300 angstrom pore size
  • Stable, predictable retention
  • Long column life

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Legal Information

apHera is a trademark of Sigma-Aldrich Co. LLC

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Khin Than Myint et al.
Analytical chemistry, 81(18), 7766-7772 (2009-08-26)
We have developed a practical method for the comprehensive analysis of polar anionic metabolites in biological samples with the use of a nano-LC/MS system. A polyamine-bonded polymer-based apHera NH2 column, which is compatible with ammonium carbonate buffer, effectively retained anionic
Marine de Person et al.
Journal of chromatography. A, 1081(2), 174-181 (2005-07-26)
A new procedure based on hydrophilic interaction chromatography coupled to tandem mass spectrometry (ionisation process by pneumatically assisted electrospray in negative ion mode), is developed and validated for the simultaneous determination of underivatised taurine and methionine in beverages rich in

Articles

Improved Analysis of Simple Sugars Using apHera™ NH2 HPLC Columns

The apHera NH2 polymeric column is an excellent choice for the separation of closely-related polar analytes such as simple sugars using HILIC chromatography.

LC/MS/MS Method for Determination of Glyphosate, AMPA, and Glufosinate in Cereals

Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.

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