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Supelco

Discovery® Glycan solid phase extraction (SPE) Cartridge

bed wt. 250 mg, volume 3 mL, pk of 54

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UNSPSC Code:
41115712
NACRES:
NB.21

product name

Discovery® Glycan SPE Tube, bed wt. 250 mg, volume 3 mL, pk of 54

material

PE frit
polypropylene

Quality Level

100

composition

bed wt., 250 mg

packaging

pk of 54

technique(s)

solid phase extraction (SPE): suitable

volume

3 mL

impurities

<5% Water content

matrix

polyamide resin base material

matrix active group

amide, poly- phase

particle size

50-160 μm

pH range

4.5-7.5(surface pH)

bulk density

0.2‑0.3 g/mL

separation technique

reversed phase

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General description

Retention Mechanism: Reversed-phase
Sample Matrix Compatibility: Aqueous or methanolic solutions

  • Polyamide Resin: Particle Size: 50-160 μm, Surf pH: 4.5-7.5, Density: 0.2-0.3 cm3/g, Water Content: < 5 %
  • Useful for extracting gylcans from aqueous solutions.
  • Used to adsorb polar compounds (-OH groups, esp. phenolic compounds) from aqueous or methanolic solutions under the reversed-phase mechanism through strong hydrogen bonding between compound hydroxyl groups and amide groups of the resin
  • Also may be used for the extraction of tannins, chlorophyll, humic acid, pharmacologically active terpenoids, flavanoids, gallic acid, catechol A, protocatechuic acid, phloroglucinol, aromatic carboxylic acids, and nitroaromatic compounds
  • Irreversibly retains quinones.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hsin-Yuan Chen et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 49(5), 1970-1986 (2018-09-21)
Uterine leiomyomas (ULs) are benign uterine tumors, and the most notable pathophysiologic feature of ULs is excessive accumulation of extracellular matrix (ECM). Fucoidan is a polysaccharide extracted from brown seaweeds that has a wide range of pharmacological properties, including anti-fibrotic
Wei Liu et al.
Glycobiology, 28(6), 363-373 (2018-03-22)
O-linked N-acetylglucosamine (O-GlcNAcylation) is an important post-translational modification on serine or threonine of proteins, mainly observed in nucleus or cytoplasm. O-GlcNAcylation regulates many cell processes, including transcription, cell cycle, neural development and nascent polypeptide chains stabilization. However, the facile identification
Weston B Struwe et al.
Cell reports, 24(8), 1958-1966 (2018-08-23)
Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes. The same bnAbs also bind to recombinant, soluble trimeric immunogens based on the SOSIP design. While SOSIP trimers are close
Qingxin Cui et al.
Frontiers in pharmacology, 9, 948-948 (2018-09-07)
Purpose: Understanding the distribution and interaction of the Traditional Chinese Medicines (TCMs) is an integral source of herbal drug discovery. An optimized radio-labeled method was explored that could conduct in situ biodistribution studies in animals. We evaluated the feasibility of
Tongqing Zhou et al.
Immunity, 48(3), 500-513 (2018-03-20)
Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit. From an HIV-1-infected donor, #74, we identified antibody VRC-PG05, which
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Articles

Solid Phase Extraction: Reversed-Phase Methodology

Reversed-phase interaction will retain most molecules with hydrophobic character; it is very useful for extracting analytes that are very diverse in structure within the same sample.

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