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Key Documents

Safety Information

55465-U

Supelco

Discovery® Glycan SPE Tube

bed wt. 50 mg, volume 1 mL, pk of 108

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1 PKG
¥4,017.87

About This Item

UNSPSC Code:
41115712
NACRES:
NB.21

¥4,017.87


Please contact Customer Service for Availability

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material

PE frit
polypropylene

composition

bed wt., 50 mg

packaging

pk of 108

technique(s)

solid phase extraction (SPE): suitable

volume

1 mL

impurities

<5% Water content

matrix

polyamide resin base material

matrix active group

amide, poly- phase

particle size

50-160 μm

pH range

4.5-7.5(surface pH)

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General description

Retention Mechanism: Reversed-phase
Sample Matrix Compatibility: Aqueous or methanolic solutions

  • Polyamide Resin: Particle Size: 50-160 μm, Surf pH: 4.5-7.5, Density: 0.2-0.3 cm3/g, Water Content: < 5 %
  • Useful for extracting gylcans from aqueous solutions.
  • Used to adsorb polar compounds (-OH groups, esp. phenolic compounds) from aqueous or methanolic solutions under the reversed-phase mechanism through strong hydrogen bonding between compound hydroxyl groups and amide groups of the resin
  • Also may be used for the extraction of tannins, chlorophyll, humic acid, pharmacologically active terpenoids, flavanoids, gallic acid, catechol A, protocatechuic acid, phloroglucinol, aromatic carboxylic acids, and nitroaromatic compounds
  • Irreversibly retains quinones.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Jarod A Zepp et al.
    Journal of immunology (Baltimore, Md. : 1950), 199(11), 3849-3857 (2017-10-27)
    This study identifies a novel mechanism linking IL-17A with colon tissue repair and tumor development. Abrogation of IL-17A signaling in mice attenuated tissue repair of dextran sulfate sodium (DSS)-induced damage in colon epithelium and markedly reduced tumor development in an
    Haley Shelley et al.
    AAPS PharmSciTech, 19(6), 2554-2563 (2018-06-28)
    Nepafenac is a nonsteroidal anti-inflammatory drug (NSAID), currently only available as 0.1% ophthalmic suspension (Nevanac®). This study utilized hydroxypropyl-β-cyclodextrin (HPBCD) to increase the water solubility and trans-corneal permeation of nepafenac. The nepafenac-HPBCD complexation in the liquid and solid states were
    Chiara Setti et al.
    European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, 71-82 (2018-06-22)
    Emulsions are known to be effective carriers of hydrophobic drugs, and particularly injectable emulsions have been successfully implemented for in vivo controlled drug release. Recently, high internal phase emulsions have also been used to produce porous polymeric templates for pharmaceutical
    Kaijie Xiao et al.
    Rapid communications in mass spectrometry : RCM, 32(2), 142-148 (2017-11-07)
    Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far. Identification and visualization tools of N-glycans
    Tongqing Zhou et al.
    Immunity, 48(3), 500-513 (2018-03-20)
    Virtually the entire surface of the HIV-1-envelope trimer is recognized by neutralizing antibodies, except for a highly glycosylated region at the center of the "silent face" on the gp120 subunit. From an HIV-1-infected donor, #74, we identified antibody VRC-PG05, which

    Articles

    This application note describes the released N-Glycan analysis of a monoclonal antibody, cetuximab, labeled with procainamide, using a BIOshell™ Glycan HPLC column.

    PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

    Reversed-phase interaction will retain most molecules with hydrophobic character; it is very useful for extracting analytes that are very diverse in structure within the same sample.

    Protocols

    A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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