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55263-U

Supelco

Protein Precipitation Filter 96-well solid phase extraction (SPE) Plate

Volume 2 mL, pk of 1

Synonym(s):

Protein Precipitation Plate

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About This Item

UNSPSC Code:
41115712
NACRES:
NB.21

product name

96-Well Protein Precipitation Filter Plate, pk of 1

packaging

pk of 1

Quality Level

technique(s)

solid phase extraction (SPE): suitable

volume

2 mL

General description

The 96-well protein precipitation filter plate is ideal for removing precipitated proteins from biological plasma/serum. The plate consists of a 0.2 μm hydrophobic graded filter/frit. Biological plasma is first added to the 96-well plate followed by a protein precipitating agent (e.g., acetonitrile). After a brief mixing step, vacuum is applied to the plate, and the filter/frit removes precipitated proteins from the sample. The resulting filtrate is ready for further processing and/or analysis.

Application


  • Determination of estrone sulfate, testosterone, androstenedione, DHEAS, cortisol, cortisone, and 17α-hydroxyprogesterone by LC-MS/MS in children and adolescents.: This study employs 96-Well Protein Precipitation Filter Plates for rapid sample preparation, significantly increasing the throughput of hormone analysis in pediatric populations. This technique enhances the accuracy and efficiency of hormonal assessments, crucial for clinical and research settings (Ankarberg-Lindgren et al., 2020).

  • Quantitative Determination of Ethyl Glucuronide and Ethyl Sulfate in Postmortem and Antemortem Whole Blood Using Phospholipid Removal 96-Well Plate and UHPLC-MS-MS.: This research demonstrates the utility of 96-Well Filter Plates in removing phospholipids from blood samples, streamlining the detection of ethyl glucuronide and ethyl sulfate, substances important for forensic and clinical toxicology (Sidqey et al., 2021).

  • Development of a CDC-certified total testosterone assay for adult and pediatric samples using LC-MS/MS.: The study highlights the application of 96-Well Protein Precipitation Filter Plates in developing a CDC-certified assay for testosterone, showing their critical role in enhancing diagnostic accuracy in clinical settings (Star-Weinstock et al., 2019).

  • Validation and application of a liquid chromatography-tandem mass spectrometric method for quantification of the drug transport probe fexofenadine in human plasma using 96-well filter plates.: This paper validates a high-throughput method for the analysis of fexofenadine, showcasing the 96-Well Filter Plates′ efficiency in clinical pharmacology and bioanalysis settings (Stanton et al., 2010).


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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

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    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What Cap Mat is used with 96-Well Protein PP Filter Plate, product 55263-U?

    96 Well Plate, product 55263-U, is a square well design, therefore, use 96 Square Well Piercable Cap Mat 575655-U.                         

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    Ask a Scientist here.

Miau-Guo Kang et al.
Journal of analytical toxicology, 43(2), 96-103 (2018-10-03)
Benzodiazepines is a large drug group used extensively to treat sleep disorders and anxiety; these drugs are frequently associated with various crimes such as murder and drug-facilitated sexual assault. With growing use and misuse of these compounds, confirmatory assays are

Articles

An article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.

This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.

Protocols

A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.

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