54817
HiTrap® Ion Exchange Cartridge
HiTrap SP (5 x 1 mL), pkg of 5 × 1 mL
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About This Item
UNSPSC Code:
12000000
Recommended Products
packaging
pkg of 5 × 1 mL
compatibility
mode of use strong cation exchange chromatography
storage temp.
2-8°C
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General description
HiTrap® SP and HiTrap® Q columns are prepacked, ready-to-use columns, suitable for syringe or peristaltic pump use (adapters included).
Recommended flow rate: 1 mL/min
Maximum back pressure: ~40 psi (3 bar)
Chemical stability: All commonly used aqueous buffers, 1 M sodium hydroxide, 8 M urea, 6 M guanidine, 70% ethanol.
HiTrap® Q: strong anion exhange Sepharose™ media for use at pH above the pI of the biomolecule (molecule will carry a negative net charge). Choose Q if the sample components are most stable above their pI.
HiTrap® SP: strong cation exhange Sepharose™ media for use at pH below the pI of the biomolecule (molecule will carry a positive net charge). Choose SP if the sample components are most stable below their pI.
Recommended flow rate: 1 mL/min
Maximum back pressure: ~40 psi (3 bar)
Chemical stability: All commonly used aqueous buffers, 1 M sodium hydroxide, 8 M urea, 6 M guanidine, 70% ethanol.
HiTrap® Q: strong anion exhange Sepharose™ media for use at pH above the pI of the biomolecule (molecule will carry a negative net charge). Choose Q if the sample components are most stable above their pI.
HiTrap® SP: strong cation exhange Sepharose™ media for use at pH below the pI of the biomolecule (molecule will carry a positive net charge). Choose SP if the sample components are most stable below their pI.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
Regulatory Information
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