SupelMIP^® SPE - TSNAs

SupelMIP SPE phases were developed by MIP Technologies AB, which is one of the leading authorities and commercial pioneers of molecular imprinted polymers for process scale separations, analytical chromatography, and sample preparation.

The SupelMIP SPE line consist s of highly cross-linked polymers that are engineered to extract a single analyte of interest or a class of structurally related analytes with an extremely high degree of selectivity. This is possible because selectivity is introduced during MIP synthesis in which a template molecule, designed to mimic the analyte, guides the formation of specific cavities or imprints that are sterically and chemically complementary to the analyte(s) of interest.

By careful design of the imprinting site, either by molecular modeling, experimental design, or screening methods, the binding cavities can be engineered to offer multiple interaction points with the analyte(s) of interest. This leads to a stronger interaction between the solid-phase and the analyte(s). As a consequence, harsher wash conditions can be tolerated during SPE methodology resulting in cleaner extracts. Because extraction selectivity is significantly improved, lower background is observed allowing analysts to achieve lower limits of detection.

Visit www.sigma-aldrich.com/supelmip to learn more.

Developed for the selective extraction of tobacco specific nitrosamines (NNN, NNK, NAT, and NAB) from human urine.

The method is highly reproducible and offers an average recovery of 80% (95% for NNK and NAT; and 70% for NNN and NAB). Detection limits of 4 pg/mL are readily achieved for urine samples using LC-MS-MS analysis.

SupelMIP is a registered trademark of Sigma-Aldrich Co. LLC